Related glossaries include Drug
Discovery & Development, Molecular
Diagnostics
Technologies Assays & screening, Cell
& tissue technologies Gene
Amplification & PCR, Molecular
Imaging,
Mass spectrometry, Microarrays
& protein chips,
Nanoscience & Miniaturization,
Sequencing, Ultrasensitivity
Technologies
term index
actuator:
A peripheral [output] device which translates electrical signals into
mechanical actions; e.g., a stepper motor which acts on an electrical signal
received from a computer instructing it to turn its shaft a certain number of
degrees or a certain number of rotations. See: servomechanism. FDA, Glossary of
Computerized System and Software Development Terminology last updated 2014 http://www.fda.gov/iceci/inspections/inspectionguides/ucm074875.htm
An actuator, the reverse of a sensor, is a device that converts an
electrical signal to an action. Actuators are further divided into three
categories: simple actuators that move valves or beams using one simple physical
law, micromotors, more complex in the design and the possibilities, and
microrobots which are the latest release in microtechnology. Materials
Research: Current Scenario and Future Projections 2003
https://books.google.com/books?id=a3EWH_vGQp0C&printsec=frontcover#v=onepage&q&f=false
a
component of a machine that is responsible for moving and controlling a
mechanism or system, for example by opening a valve.
In simple terms, it is a "mover". An actuator requires a control signal
and a source of energy. The control signal is relatively low energy and
may be electric voltage or current, pneumatic or hydraulic pressure, or
even human power. Its main energy source may be an electric
current, hydraulic
fluidpressure, or pneumatic pressure.
When it receives a control signal, an actuator responds by converting the
signal's energy into mechanical motion. An actuator is the mechanism by
which a control system acts upon an environment. Wikipedia accessed
2018 Nov 8
https://en.wikipedia.org/wiki/Actuator Narrower term: biosensor Broader terms: sensors,
transducers
amplification: See signal amplification, target amplification (and gene
amplification).
avidin:
Protein found in raw egg white which binds to
biotin (etymology is from its avidity to biotin). The interaction of biotin and
Avidin or Streptavidin has been exploited for use in many protein and nucleic
acid detection and purification methods. Because the biotin label is stable and
small, it rarely interferes with the function of labeled molecules enabling the
Avidin-biotin interaction to be used for the development of robust and highly
sensitive assays. Thermo Fisher Avidin-Biotin interaction
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/avidin-biotin-interaction.html
bioluminescence:
Luminescence produced by living systems. IUPAC
Photochemistry biosensor: A device that uses specific biochemical reactions
mediated by isolated enzymes, immunosystems, tissues, organelles or whole
cells to detect chemical compounds, usually by electrical, thermal or optical
signals. IUPAC Bioinorganic Narrower terms: electrochemical biosensor, spore
based biosensor Related term: sensor web
biotinylation:
the
process of covalently attaching biotin to
a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and
is unlikely to perturb the natural function of the molecule due to the small
size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with
an extremely high affinity, fast on-rate, and high specificity, and these
interactions are exploited in many areas of biotechnology to isolate
biotinylated molecules of interest. Biotin-binding to streptavidin and avidin is
resistant to extremes of heat, pH and proteolysis, making capture of
biotinylated molecules possible in a wide variety of environments. Also,
multiple biotin molecules can
be conjugated to
a protein of interest, which allows binding of multiple streptavidin, avidin or neutravidin protein
molecules and increases the sensitivity of detection of the protein of interest.
There is a large number of biotinylation reagents available that exploit the
wide range of possible labelling methods. Due to the strong affinity between
biotin and streptavidin, the purification of biotinylated proteins has been a
widely used approach to identify protein-protein interactions and
post-translational events such as ubiquitylation[1] in
molecular biology. Wikipedia accessed 2018 Sept 18
https://en.wikipedia.org/wiki/Biotinylation
To label a probe with biotin.
blinking: See under green fluorescent protein
Catalyzed Reporter Deposition CARD:
See Tyramide Signal
Amplification TSA.
chemiexcitation:
Generation, by a chemical reaction, of electronically
excited molecular entities from reactants in their ground electronic states.
IUPAC Photo
chemiluminescence: Emission of light as a result of a chemical
reaction without an apparent change in temperature. MeSH, 1993
Luminescence arising from chemiexcitation. IUPAC Photochemistry
colorimetry:
The methods used to measure color and to define
the results of the measurements. Photonics competitive hybridization: Gene
amplification & PCR
detection: The Oxford English Dictionary points out that
detecting involves finding what is otherwise apt to elude notice, particularly
that which is "artfully concealed". Narrower terms: gated
detection; Ultrasensitivity:
single molecule
detection
detector technologies: Include direct detection, electrochemical, fluorescence,
fluorescence
polarization, colorimetry, mass spectrometry,
luminescence, optical, primer
extension and minisequencing. Michael Phillips, CHI Nucleic
Acids Technologies conference, June 7-9, 2000 Detector instrumentation
includes CCD cameras and lasers.
differential labeling:
When
comparing the proteomes of two cell states (e.g. diseased vs. normal), gel- to-
gel variability in spot position and protein yield often places the results of
such experiments in question. Differential labeling enables one to analyze both states on a single gel,
thus enabling direct comparison of protein levels. In this method, cells are
treated with normal media, or media enriched in 15N. Corresponding
proteins from each state will migrate to the same location on the gel, but
analysis by mass spectrometry will distinguish the metabolically labeled
peptides and thus quantify the two sets of proteins separately. This can have
significant impact on reproducibility when comparing experiments.
dyes:
Chemical substances that are used to stain and color other
materials. The coloring may or may not be permanent. Dyes can also be used as
therapeutic agents and test reagents in medicine and scientific research. MeSH,
1963. Related terms: labels, reagents Narrower term:
spectroscopic dyes
electrochemical biosensor:
A self- contained integrated device,
which is capable of providing specific quantitative or semi- quantitative
analytical information using a biological recognition element (biochemical
receptor) which is retained in direct spatial contact with an electrochemical
transduction element. Because of their ability to be repeatedly calibrated,
we recommend that a biosensor should be clearly distinguished from a bioanalytical
system, which requires additional processing steps, such as reagent
addition. A device which is both disposable after one measurement, i.e., single
use, and unable to monitor the analyte concentration continuously or after rapid
and reproducible regeneration should be designated a single use biosensor. IUPAC
Commission on Electroanalytical Chemistry, Electrochemical Biosensors:
Recommended Definitions and Classification, 1999
https://www.iupac.org/publications/pac/71/12/2333/index.html
Broader term: biosensor
electrochemiluminescence: See Electrogenerated Luminescence
ECL.
IUPAC
Photo electrochemistry:
The study of chemical changes resulting from
electrical action and electrical activity resulting from chemical changes. MeSH,
1966 Related terms: electrochemiluminescence, Electrogenerated
Luminescence ECL, electroluminescence
Electrochemistry Dictionary, 1000 definitions
http://knowledge.electrochem.org/ed/dict.htm
Electrochemistry
Encyclopedia
http://knowledge.electrochem.org/encycl/
Electrogenerated Luminescence
ECL:
Luminescence produced by electrode reactions. Also called
electroluminescence or electrochemiluminescence. IUPAC Photo electroluminescence:
See electrogenerated chemiluminescence. IUPAC
Photo electronic nose:
a device intended to
detect odors or flavors.
Over the last decades, "electronic sensing" or "e-sensing" technologies
have undergone important developments from a technical and commercial
point of view. The expression "electronic sensing" refers to the
capability of reproducing human senses using sensor arrays and pattern
recognition systems. Since 1982,[2] research
has been conducted to develop technologies, commonly referred to as
electronic noses, that could detect and recognize odors and flavors. The
stages of the recognition process are similar to human olfaction and
are performed for identification, comparison, quantification and
other applications, including data
storage and retrieval. However, hedonicevaluation
is a specificity of the human nose given that it is related to subjective
opinions. These devices have undergone much development and are now used
to fulfill industrial needs. Wikipedia accessed 2018 Oct 26
https://en.wikipedia.org/wiki/Electronic_nose
A device used to detect
airborne odors, gases, flavors, volatile substances or vapors. MeSH Year
introduced: 2013
electronic
tongues: For liquid analysis, based on the organizational principles
of biological sensory systems, developed rapidly during the last decade. ... The
exciting possibility of establishing a correlation between the output from an
electronic tongue and human sensory assessment of food flavour, thereby enabling
quantification of taste and flavour, is described. Application areas of
electronic tongue systems including foodstuffs, clinical, industrial, and
environmental analysis are discussed in depth. Y. Vlasov et. al.,
"Electronic tongues and their analytical application" Analytical
Bioanalytical Chem. 2002 Jun;373 (3): 136- 146, June 2002 Broader term:
sensors
excitation emission
spectrum: The three-dimensional spectrum generated by
scanning the emission
spectrum at incremental steps of excitationwavelength ( axis
= emission wavelength, axis
= excitation wavelength, axis
= emission flux) is called a (fluorescence, phosphorescence)
excitation-emission
spectrum (or EES). The spectra are particularly useful for investigating
samples containing more than one emitting species. Corrected EES are obtained if
(a) the emission is corrected for instrumental response with wavelength,
and (b) the exciting radiation flux in photons is held constant for all
excitation wavelengths. IUPAC Gold Book
https://goldbook.iupac.org/html/E/E02249.html Narrower terms: chemiexcitation,
photoexcitation Related terms: quencher, quenching, scintillation
Fluorescence Recovery After Photobleaching FRAP:
A method used to study the lateral movement of MEMBRANE
PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser
light and the amount of time necessary for unbleached fluorescent marker- tagged
proteins to diffuse back into the bleached site is a measurement of the cell
membrane's fluidity. The diffusion coefficient of a protein or lipid in the
membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).
MeSH 2003
fluorescence:
Luminescence which occurs essentially only during
the irradiation of a substance by electromagnetic radiation. IUPAC Compendium
The property of emitting radiation while being irradiated. The radiation
emitted is usually of longer wavelength than that incident or absorbed,
e.g., a substance can be irradiated with invisible radiation and emit visible
light. X-ray fluorescence is used in diagnosis. MeSH Narrower terms: FISH Fluorescence In Situ Hybridization, Fluorescence Resonance Energy Transfer
FRET, green fluorescent protein GFP; FRAP Fluorescence Recovery After
Photobleaching, fluorescence polarization, LIF Laser Induced Fluorescence Related
terms: fluorophore
Fluorescence In Situ Hybridization: See Gene
Amplification & PCR FISH
Fluorescence Resonance Energy Transfer FRET:
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT
DYES with overlapping emission and absorption spectra, which is used to indicate
proximity of labeled molecules. This technique is useful for studying
interactions of molecules and PROTEIN FOLDING. MeSH 2003
Narrower term: single-pair FRET spFRET
fluorophore:
May refer to either the fluorescent label or marker,
or to the atoms which make the label fluorescent.
green fluorescent protein GFP:
As a label for reporting cellular events in situ has been explored by a
large number of laboratories. GFP and its mutants offer a powerful advantage as
clonable markers for use in living tissue. However, photoisomerization
and flickering of the emission signal ('blinking') create a challenge in single
molecule experiments for both types of probe. Studies are in progress by
W.E. Moerner and others (for example, see 6,7)
to understand the basis for the long- lived dark states that lead to
fluctuations in the emission spectra from these molecules, and to develop
improved probes with reduced photoisomerization and blinking. [NIGMS
Single Molecule Detection and Manipulation Workshop" Single Molecule Fluorescence of Biomolecules and Complexes Protein Folding
April
17-18, 2000] http://www.nigms.nih.gov/news/reports/single_molecules.html#examples
Broader term:
fluorescent proteins
Isotope Coded Affinity Tags (ICAT): See under differential labeling
in situ hybridization ISH: Gene
amplification & PCR
indirect detection:
See under reporter gene
isothermal:
Isothermal conditions are important in immunoassays,
immunohistochemistry, in
situ amplification and in cell- based assays, in which one wants to retain the
morphology and viability of the cells. The relative simplicity of an isothermal
reaction also indicates greater utility for point of care diagnostic
applications. Having a constant temperature.
label: A marker, tag or indicator distinguishable by the observer
but not by the system and used to identify a tracer. IUPAC Nomenclature
for Radioanalytical Chemistry,
Pure & Applied Chemistry: 2514- 2576, 1994
https://www.iupac.org/publications/pac/pdf/1994/pdf/6612x2513.pdf
There is a need to develop better methods for inserting site- specific labels
in the samples for detection, as well as mechanical handles for manipulation. Site-
directed mutagenesis, approaches using chimeras, clonable tags, reporter
genes, protection/ deprotection protocols, and protein modification using
derivatized amines and thiols, such as His tags, are currently used, but
flexibility in the placement of chemical handles in the sample remains a
limitation. NIGMS, Single Molecule Detection and Manipulation Workshop
"Single Molecule Fluorescence of Biomolecules and Complexes Protein
Folding, April 17-18, 2000 http://www.nigms.nih.gov/news/reports/single_molecules.html#examples
Narrower
terms: nanolabels, biotin, differential labelling, ICAT, optical tagging, streptavidin, target labelling
Related terms: assays, dyes, reagents, staining and labeling, target labelling, tracer;
Ultrasensitivity
label free:
In the journey of a molecule
from its origins in a compound library to candidate drug status, a large variety
of profiling must occur to define activity, selectivity, potency, adverse
effects, pharmacology and in vivo efficacy. Advances in biophysical methods that
can analyse drug interactions with a molecular target, a whole cell, or even ex
vivo tissue have enabled many of these studies to be carried out without the
need for reporter-based or ‘labelled’ assays. Label-free screening in
high-throughput mode can be used as a pathway independent screening tool with
whole cells, or in low-throughput mode with individual receptors to define
interaction kinetics and thermodynamics. European Pharmaceutical review,
What is label free screening and why use it in drug discovery?
https://www.europeanpharmaceuticalreview.com/article/16213/what-is-label-free-screening-and-why-use-it-in-drug-discovery/
luminescence: The property of giving off light without emitting
a corresponding degree of heat. It includes the luminescence of inorganic
matter or the bioluminescence of human matter, invertebrates and other
living organisms. MeSH
Spontaneous emission of radiation from an electronically or vibrationally
excited species not in thermal equilibrium with its environment. IUPAC
Compendium
Narrower terms: bioluminescence, chemiluminescence,
electrochemiluminescence, Electrogenerated Luminescence ECL.
luminometer:
Light measuring instrument.
molecular biosensors: Devices
of molecular size that are designed for sensing different analytes on the basis
of biospecific recognition. They should provide two coupled functions - the
recognition (specific binding) of the target and the transduction of information
about the recognition event into a measurable signal. Fluorescence sensing of
intermolecular interactions and development of direct molecular biosensors
Danièle Altschuh 1 *, Sule Oncul 2, Alexander P.
Demchenko 2 Journal of Molecular Recognition: `9 (6): 459-477
published online 6 Nov 2006 http://www3.interscience.wiley.com/cgi-bin/abstract/113445627/ABSTRACT?CRETRY=1&SRETRY=0
.
molecular combing:
A method ... which can straighten and align
molecules of genomic DNA on a solid surface. The technology also includes a
battery of novel statistical methods developed for analyzing the large amounts
of data obtained from FISH analyses made on individual DNA molecules.
Wikipedia
https://en.wikipedia.org/wiki/Chromosome_combing also known as
molecular combing or DNA combing nanolabel:
A novel double-codified nanolabel (DC-AuNP) based on gold nanoparticle (AuNP)
modified with anti-human IgG peroxidase (HRP)-conjugated antibody is reported.
It represents a simple assay that allows enhanced spectrophotometric and
electrochemical detection of antigen human IgG as a model protein. Ambrosi A,
Castañeda MT, Killard AJ, Smyth MR, Alegret S, Merkoçi A. Double-codified gold
nanolabels for enhanced immunoanalysis, Analytical Chemistry 2007 Jul
15;79 (14): 5232- 5240. Epub 2007 Jun 19. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17579481&ordinalpos=1&itoo...
photoexcitation:
Luminescence arising from photoexcitation. IUPAC Photo
primed in situ labeling:
A technique that labels specific sequences in whole chromosomes by
in situ DNA chain elongation or PCR (polymerase chain
reaction). MeSH, 1999
Quantum
Dots (QD): very small semiconductor particles,
only several nanometres in
size, so small that their optical and electronic properties differ from
those of larger LED particles. They are a central theme in nanotechnology.
Many types of quantum dot will emit light of
specific frequencies if electricity or light is applied to them, and these
frequencies can be precisely tuned by changing the dots' size,[1][2]shape
and material, giving rise to many applications. In the language of materials
science, nanoscale semiconductor
materials tightly confine either electrons or electron
holes. Quantum dots are also
sometimes referred to as artificial atoms, a term that emphasizes that a
quantum dot is a single object with bound,
discrete electronic states, as is the case with naturally occurring atoms or molecules.[3][4]
Wikipedia accessed 2018 Nov 11
https://en.wikipedia.org/wiki/Quantum_dot
Biology applications
https://en.wikipedia.org/wiki/Quantum_dot#Biology
quencher:
A molecular entity that deactivates (quenches) an excited state of another molecular
entity, either by energy transfer, electron transfer, or by a chemical mechanism.
IUPAC Photo
quenching:
1. Arresting the course of a chemical reaction by
chemical or physical means. (in photochemistry) 2. The deactivation of
an excited molecular entity intermolecularly by an external environmental
influence (such as a quencher) or intramolecularly by a substituent through
a nonradiative process. 3. (in radiation chemistry) The process of inhibiting
continuous or multiple discharges following a single event in certain types
of radiation detectors. [IUPAC Compendium]
reactant:
A substance that is consumed in
the course of a chemical reaction. It is sometimes known, especially in
the older literature, as a reagent, but this term is better used in a more
specialized sense as a test substance that is added to a system in order to
bring about a reaction or to see whether a reaction occurs (e.g. an analytical
reagent). IUPAC Compendium Related term: reagents
reagents: Substances used for the detection, identification,
analysis, etc. of chemical, biological, or pathologic processes or conditions.
.. Reagents are substances used for the detection or determination of another
substance by chemical or microscopical means, especially analysis. Types
of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and
colorimetric reagents. MeSH ‘indicators and reagents’
Related
term: reactant
scintillation:
Burst of luminescence of short duration caused
by an individual energetic particle. IUPAC Radioanalytical
sensitivity:
100% sensitivity = 100% true positives, 0% false
positives.
sensor:
In
the broadest definition, a sensor is
a device, module, or subsystem whose purpose is to detect events or
changes in its environment and send the information to other electronics,
frequently a computer
processor.
Wikipedia accessed 2018 March 22
https://en.wikipedia.org/wiki/Sensor
Related terms: actuators, labels, nanoparticles, sensor model
language, transducers Narrower terms: biosensor, electrochemical biosensors, electronic biosensor, electronic nose,
electronic tongues, molecular biosensors, nanosensors, neurally inspired sensor,
spore based biosensors
sensor model language
SensorML:
Sensor
Model Language (SensorML) for In-situ and Remote Sensors. Edited by Mike
Botts (University of Alabama in Huntsville). Open GIS Discussion Paper [not
an adopted standard]. Open GIS Consortium Inc. Issued by the OGC Natural
Resources and Environment (NRE) Working Group. Publication Date: 2002-12-20.
Reference number: OGC 02-026r4. Version 0.7. 118 pages. With associated XML
Schemas. http://xml.coverpages.org/ni2003-01-31-b.html
With definitions of sensors.
sensor web:
An independent network of wireless,
intra- communicating sensor pods, deployed to monitor and explore a limitless
range of environments. This adaptable instrument can be tailored to whatever
conditions it is sent to observe. Jet Propulsion Lab, NASA "Sensor Web Project" http://sensorwebs.jpl.nasa.gov/
Sensors Global Summit
2018 Dec 10-12 San Diego CA Emerging technological innovations
enabled by next generation sensors will define the landscape for the
future commercialization of life changing products. The exponential growth
of the global sensors market and the demand for next generation integrated
systems is expected to reach $154 billion by 2020, the latest
technological advancements of real-world applications within healthcare
and emerging markets
http://www.sensorsglobalsummit.com/
signal amplification:
PCR
signal to noise:
Ratio which can interfere with detection. Can
also refer to data analysis. Biological data is often very "noisy".
This is particularly seen when trying to look at low abundance biomolecules.
signals:
Produced by dyes, fluorescence or radioactivity.
(Non- radioactive materials, because of disposal and other problems, and
improvements in other technologies) are increasingly in demand. Related terms:
detection, reagents, actuators
single cell, single molecule: Ultrasensitivity
single-pair FRET
spFRET: Designed to overcome the averaging effects of
ensemble studies because measurements are made on single molecules freely
diffusing in solution. This method limits the observation period to the
diffusion time of each molecule through the focal spot of a laser on the order
of a few hundred milliseconds, but it permits the rapid gathering of data at single-
molecule resolution on a large number of molecules in a short time
period. SpFRET can be used to study intramolecular conformational changes by
placing the donor and acceptor fluorescent tags on two different sites of the
same macromolecule, or alternatively, intermolecular interactions can be studied
by attaching the donor and acceptor tags to two different macromolecules.
NIGMS, Single Molecule Detection and Manipulation Workshop
"Single Molecule Fluorescence of Biomolecules and Complexes
Protein Folding April 17- 18, 2000 http://www.nigms.nih.gov/news/reports/single_molecules.html#examples
spectroscopic dyes:
These dyes—in particular, nanoparticles
— are
emerging as alternatives to fluorescent dyes. Because the emission spectra of
nanoparticles vary according to these particles’ specific size and shape,
these nanostructures can be used in multicolor detection formats, potentially
offering much greater multiplexing than is currently achievable. The fact that
chemists have been able to create a great variety of structures (and properties)
in nanoparticles suggests that these particles might be more "finely tunable"
than organic dyes, allowing better results from biological assays
CHI High-
Content Analysis Market Outlook report, 2004
staining and labeling:
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
MeSH, 2001 Related terms: label, tags
streptavidin:
A 60 kD extracellular protein of Streptomyces
avidinii with four high-affinity biotin binding sites. Unlike
AVIDIN, streptavidin has a near neutral isoelectric point and
is free of carbohydrate side chains. MeSH, 1998
Surface Plasmon Resonance SPR:
Technologies overivew
TSA Tyramide Signal Amplification:
TSA - also known as Catalyzed
Reporter Deposition (CARD) - is a signal- amplification technology designed
to enhance detection sensitivity in DNA arrays, in situ hybridization
(ISH) assays, and other applications. Kerstens HM, Poddighe PJ, Hanselaar AG.
"A novel in situ hybridization signal amplification method based on
the deposition of biotinylated tyramine." Journal of Histochemistry
& Cytochemistry. 1995. 43:347-352.
tag: See label, capture tag, Isotope Coded Affinity Tags ICAT,
optical tagging. This is different from tagging for information
retrieval
target labelling:
Drug & disease Targets
tracer:
Labelled members of a population used to measure certain
properties of that population. IUPAC Radioanalytical
transducer:
A transducer is a device that converts energy
from one form to another. Usually a transducer converts a signal in
one form of energy to a signal in another.[1]
Transducers are often employed at the boundaries of automation, measurement,
and control systems,
where electrical signals are converted to and from other physical
quantities (energy, force, torque, light, motion, position, etc.). The
process of converting one form
of energy to another is known as transduction.[2]
Wikipedia accessed 2018 Nov 9 http://en.wikipedia.org/wiki/Transducers
Narrower terms: actuators, sensors
Labels Signaling Detection Resources
IUPAC
Commission on Electroanalytical Chemistry, Electrochemical Biosensors:
Recommended Definitions and Classification, 1999
https://www.iupac.org/publications/pac/71/12/2333/index.html
IUPAC Glossary of Terms used in Photochemistry, 3rd ed. 2006 http://iupac.org/publications/pac/79/3/0293/
IUPAC Nomenclature
for Radioanalytical Chemistry,
Pure & Applied Chemistry: 2514- 2576, 1994
https://www.iupac.org/publications/pac/pdf/1994/pdf/6612x2513.pdf
IUPAC
Terminology for Biomolecular Screening 2011 http://iupac.org/publications/pac/83/5/1129/
Molecular Probes the Handbook
http://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook.html
How
to look for other unfamiliar terms
IUPAC definitions are reprinted with the permission of the International
Union of Pure and Applied Chemistry.
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